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Image Search Results
Journal: The Journal of Cell Biology
Article Title: Rap1 controls cell adhesion and cell motility through the regulation of myosin II
doi: 10.1083/jcb.200607072
Figure Lengend Snippet: Subcellular localization of GFP-Phg2 and morphology of phg2 -null cells. (A) Translocation of GFP-Phg2 to the cell cortex in response to cAMP stimulation. The fluorescence intensity on the plasma membrane fraction or in the cytosol fraction was quantitated using SimplePCI Imaging Systems. The graphs represent mean values of 10 cells from two separate experiments. (B) Localization of GFP-Phg2 in the migrating cells. The arrow indicates the direction of movement. (C) Morphology of phg2 -null cells. Vegetative cells were plated in HL5 growth medium or Na/K phosphate buffer on a plate with a hole covered by a glass coverslip and were imaged. phg2 -null cells show lamellipodia-like (white arrows) or filopodia-like structures (black arrows) compared with wild-type cells.
Article Snippet: Images were captured using
Techniques: Translocation Assay, Fluorescence, Clinical Proteomics, Membrane, Imaging